Disturbance in the biological cycles of P. The activity of enzymes (Phenoloxidase and Superoxide dismutase) and immune genes (Defensin, Spaetzle, Cecropin, Lysozyme, and Hemolin) did vary at different time points (24, 48, 72 and 96 h) after exposure to M. However, no statistically significant effect on hemocyte concentration was observed. anisopliae resulted in high pest mortality by killing 93% of larvae. anisopliae, a naturally existing soil-borne entomopathogenic fungus. The study was designed to investigate the mortality, development, and immune responses of P. Three different species of entomopathogenic fungi were evaluated before the selection (high larval mortality and least LC 50) of Metarhizum anisopliae. Besides having no hazardous effects on the environment, these entomopathogens are alternatives to synthetic insecticides that can control notorious insect-like Plutella xylostella, a destructive pest of cruciferous crops. AbstractĮntomopathogenic fungi are naturally existing microbes, that can serve as a key regulator of insect pests in integrated pest management strategies. This information will also reinforce the development of policies for biocontrol-based pest management. anisopliae can become a potent prospect for the control of this pest. Moreover, the fungus negatively impacted the development of the host by reducing the life span and egg laying ability. ![]() The expression of immune-related genes (Defensin, Spaetzle, Cecropin, Lysozyme, and Hemolin) varied on different time points. The fungus gradually weakened the enzyme activities as the time progressed, indicating that physiological attributes of host were adversely affected. More precisely, after infection, superoxide dismutase (SOD) and phenoloxidase (PO), two major enzymes involved in immune response, were studied at different time points. ![]() Based on which the fungus was selected to study the host-pathogen immune interactions. Metarhizium anisopliae proved to be the most effective by killing more than 90% of the population. In this study, the virulence of three species of entomopathogenic fungi were tested. Integrated pest management (IPM) strategies, largely involve the use chemical pesticides which are harmful for the environment and human health. The diamondback moth, Plutella xylostella, is a destructive pest of cruciferous crops worldwide.
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